RNA

Part:BBa_K411001:Design

Designed by: Po-Hsiang Liao   Group: iGEM10_NYMU-Taipei   (2010-10-15)

Theophylline-inducible Riboswitch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This riboswitch has the following characteristics:

  • The inducer (Theophylline) cannot metabolize in E. coli.
  • Both the inducer (Theophylline) and the riboswitch itself does not naturally occur in E. coli .
  • It did not originally have EcoRI, XbaI, SpeI, and PstI cutting sites, so no point mutations were needed.

See [http://2010.igem.org/Team:NYMU-Taipei NYMU-Taipei's Design page]

Source

Synthesized directly from two primers:

  • forward primer : 5' - gaattcgcggccgcttctagag ggtgataccagcatcgtcttgatgcccttggcag - 3'
  • reverse primer : 5' - ctgcagcggccgctactagta cttgttgtcttgcagcggggtgctgccaagggcatcaagac - 3'

References